Why RFLP and MICR Are Not Always the Best Assay Formats for Chemical Analysis

With the increased popularity of RFLP testing there has been an increase in questions concerning the benefits of a lamp vs. a PCR. The truth is both are great tools when it comes to DNA analysis and have their own place in the DNA process. However the real question should be whether the differences between the two methods are significant enough to warrant investing in one over the other. Here is a quick comparison.

The primary difference between the lamp and RFLP testing is that the lamp operates at constant temperature (usually 60-65C). This allows for more sensitive and effective measurements, particularly with shorter strands of DNA. RFLP, on the other hand, operates at certain temperatures depending on the length of the strand. For example, if the strand is shorter it will typically operate at lower temperatures than a strand that is longer due to the larger surface area of the DNA probe. If the temperature is too low, an error can be made when detecting micro-movements which can invalidate the results from the RFLP tests. It is also important to note that a strand length of less than 75 atoms can be amplified by RFLP.

In addition to the difference in temperature between the two methods, there are also some significant differences between the types of probes used in these processes. While most medical laboratories utilize mssA and rtps probes, there are a small percentage of labs that utilize pH detergent instead. Using mssA or rtps can help detect short reads that are not typical to any standard technique. Furthermore, using pH detergent instead of mssa can help remove certain contaminates from the samples, especially those that are too large in size to be contained by standard filters or disks.

There are additional differences between the two types of RFLP testing, which we will discuss later in this article. However, for our purposes here, we will focus on the main differences between the two and why the MICR type is preferred in clinical applications and the RFLP test is preferred for standard applications. First we will look at the differences in the DNA probe that is being used. When a DNA sample is under investigation, a specific sequence of bases will be used in order to generate the read.

This makes the RFLP assay much more sensitive than the MICR assay in a number of laboratory applications. Next we will look at the key difference between the two in terms of the thermal cycling processes that are employed to give reliable results. One of the main reasons why the RFLP test has been used in the laboratory for so long is because it can give more reliable results than the MICR based lamp vs pcr test. The reason for this is due to the fact that the thermal cycler allows for a higher degree of control over the rate of heating of the DNA sample.

For this reason, the MICR-based assays are commonly used in routine laboratory investigations where high throughput and/or sensitive measurements are desired. However, the RFLP process is used specifically when certain forms of molecular information must be amplified, cooled and diluted at very high temperatures. Due to the cost-effective and sensitive nature of the RFLP process, it is highly utilized in workflow automation and industrial laboratories where high throughput and sensitivity are required.